TABLE 6.
Estimated richness of E. coli populations obtained from 5 representative sampling sites over the study perioda
| Method | Site | r2b | No. of genotypes: |
% of predicted genotypes that were detected | No. of isolates required to capture 50% of predicted genotypes (mean ± SD)e | |
|---|---|---|---|---|---|---|
| Predicted (mean ± SD)c | Detectedd | |||||
| BOX-PCR | MST-1 | 0.9999 | 5,794 ± 196 | 1,196 | 21 | 14,384 ± 578 |
| MST-5 | 0.9999 | 6,964 ± 207 | 1,621 | 23 | 19,007 ± 685 | |
| MST-6 | 0.9999 | 6,138 ± 190 | 1,479 | 24 | 17,282 ± 654 | |
| MST-9 | 0.9999 | 3,970 ± 133 | 888 | 22 | 13,697 ± 549 | |
| MST-15 | 0.9998 | 1,220 ± 69 | 325 | 27 | 4,589 ± 325 | |
| ERIC-PCR | MST-1 | 0.9999 | 10,295 ± 514 | 1,755 | 17 | 19,614 ± 1,123 |
| MST-5 | 0.9998 | 9,599 ± 385 | 2,152 | 22 | 19,390 ± 937 | |
| MST-6 | 0.9998 | 10,374 ± 413 | 2,093 | 20 | 20,985 ± 985 | |
| MST-9 | 0.9998 | 6,491 ± 311 | 1,470 | 23 | 13,294 ± 769 | |
| MST-15 | 0.9998 | 2,083 ± 172 | 509 | 24 | 5,050 ± 509 | |
a
The total numbers of water samples and E. coli isolates obtained from each site are given in Table 2.
b
Coefficient of determination of the goodness of fit to the Michaelis-Menten equation.
c
The Vmax parameter in the Michaelis-Menten equation.
d
The number of genotypes detected in the rarefaction analysis is used to estimate the percentage of total community diversity that was captured in the collection.
e
The Km parameter in the Michaelis-Menten equation.