TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Description | Source |
|---|---|---|
| Strains | ||
| Bradyrhizobium sp. strain JS329 | 5-Nitroanthranilic acid degrader | This study |
| Escherichia coli EPI300 | Host strain for pJS800, pJS801, and pJS802 | Epicentre (Madison, WI) |
| Plasmids | ||
| pCC1FOS | Cmr; 8.1-kb fosmid vector for construction of the genomic library | Epicentre (Madison, WI) |
| pJS800 | Cmr; 40-kb pCC1FOS containing naaA and naaB from JS329 (5NAA+ 5NSA+) | This study |
| pJS801 | Cmr Kmr; transposon Tn5 insertion into naaA of pJS800 (5NAA− 5NSA+) | This study |
| pJS802 | Cmr Kmr; transposon Tn5 insertion into naaB of pJS800 (5NAA+ 5NSA−) | This study |