TABLE 2.

Effect of additions on transformation of 5NSA by ultrafiltered extract from 5NAA-grown cells^a

Addition(s)	Relative rate (%)
Fe2+	100
NADH	23.3 ± 1.7
Fe3+	9.4 ± 1.2
Fe2+, NADH	87.7 ± 2.2
Fe2+, NADH, FAD	102.1 ± 0.6
Fe2+, NADH, FMN	90.4 ± 0.8
Fe2+, NADH, DTT	70 ± 14
No cofactor	17 ± 2.4

^aCells were incubated at 30°C with shaking. The concentrations were as follows: for Fe²⁺, 50 μM; for Fe³⁺, 50 μM; for NADH, 150 μM; for FMN, 10 μM; for FAD, 10 μM; and for DTT, 1 mM.