TABLE 1.
Candidate reference genes and genes of interest
| Gene group and name | Molecular function | Primer name and sequencea | Amplicon length (bp) | Efficiencyb | r2 |
|---|---|---|---|---|---|
| Candidate reference genes | |||||
| gyrB | DNA gyrase, subunit B | F: CCAGGTAAATTAGCCGATTGC | 121 | 95 | 0.994 |
| R: AAATCGCCTGCGTTCTAGAG | |||||
| ftsZ | Cell division protein | F: ATCCAAATCGGTGAAAAATTAACAC | 121 | 96 | 0.998 |
| R: CCATGTCTGCACCTTGGATTG | |||||
| hu | DNA-binding protein | F: TTTACGTGCAGCACGTTCAC | 125 | 92 | 0.999 |
| R: AAAAAGAAGCTGGTTCAGCAGTAG | |||||
| rplD | 50S Ribosomal protein L4 | F: TGAAAGATAATGCTGAGCGTAAAG | 121 | 104 | 0.998 |
| R: ACAATCCGTGCTCCACAATG | |||||
| recA | Recombinase A | F: GGGAGACACTCACGTTGGTTTAC | 121 | 86 | 0.996 |
| R: AACTTTTTCACGAATTTGGTTGATG | |||||
| sodA | Superoxide dismutase | F: TTAGGTAATAAGCGTGTTCCCATAC | 125 | 83 | 0.994 |
| R: GGCTTGGTTAGTCGTAAACAATG | |||||
| gap | Glyceraldehyde-3-phosphate | F: AGTGAACCAGATGCAAGCAAATTAC | 121 | 96 | 0.999 |
| dehydrogenase | R: TTTTAGCGCCTGCTTCAATATG | ||||
| rpoB | RNA polymerase beta chain | F: GCGAACATGCAACGTCAAG | 121 | 98 | 0.999 |
| R: GACCTCTGTGCTTAGCTGTAATAGC | |||||
| pta | Phosphate acetyltransferase | F: AAAGCGCCAGGTGCTAAATTAC | 121 | 98 | 0.994 |
| R: CTGGACCAACTGCATCATATCC | |||||
| tpi | Triose phosphate isomerase | F: TTCTGAACGTCGTGAATTATTCC | 121 | 100 | 0.996 |
| R: TTCACGCTCTTCGTCTGTTTC | |||||
| Enterotoxin genes | |||||
| sea | Enterotoxin A | F: TCAATTTATGGCTAGACGGTAAACAA | 93 | 101 | 0.994 |
| R: GAAGATCCAACTCCTGAACAGTTACA | |||||
| sed | Enterotoxin D | F: AAACGTTAAAGCCAATGAAAACA | 150 | 98 | 0.997 |
| R: TGATCTCCTGTACTTTTATTTTCTCCTA |
a
F, forward; R, reverse.
b
PCR amplification efficiency was determined from the standard curves, using the formula 10(−1/slope). For the calculations, the base of the exponential amplification function is used (e.g., 1.94 means 94% amplification efficiency).