Electrophoresis of amplified products by the multiplex PCR. Lane A, DNA ladder mix (Fermentas); lanes B to D, human clinical strains of biotype 1 (CECT 529T, YJ016, and CECT 5168); lanes E to H, environmental isolates of biotype 1 (MLT 362, CECT 4606, CECT 5165, and CECT 4867); lanes I to L, eel and human isolates of biotype 2 serovar E (CECT 4999, CECT 4866, CCUG 38521, and CIP 8190); lanes M and N, eel isolates of biotype 2 serovar A (CECT 5768 and CECT 5689); lanes O and P, eel isolates of serovar I (95-8-161 and 95-8-6); lanes Q and R, human isolates of biotype 3 (vv12 and 11028); and lanes S to W, other vibrio species (V. alginolyticus, V. anguillarum, V. cholerae, V. natriegens, and V. parahaemolyticus).