FIG. 4.

Comparison of the different methods for an internal validation set of seven composite images. (A) Measurement of cumulative filament length by manual filament tracing, the automated method, and the line-intercept method. The error bars for the manual measurements indicate the standard deviations of four replicates. (B) Filament numbers as quantified by manual counting and by the automated method. The error bars for the manual measurements indicate the standard deviations of four replicates. (C) Linear regression of 100 selected filaments of different lengths from the seven images that were individually measured manually and by the automated approach.