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. 2009 Dec 28;76(5):1661–1668. doi: 10.1128/AEM.01468-09

FIG. 4.

FIG. 4.

Open reading frames present in the sequence of the pSKK2/EAB150 insert. Two ORFs that start with the CTG initiation codon, preceded by appropriate sequences that resemble the ribosome-binding site, were found in this intergenic region of 124 bases. Those ORFs were 36 and 15 codons long. Frame shift mutations were introduced into these ORFs to test the possibility that either of the two ORFs encodes a small protein (asterisks represent the bases deleted to release a mutant of each ORF).