TABLE 1.
Sequences of the primers and probes used in this study
| Primers and probes | Primer and probe sequence (5′→3′) | Size of amplicon (bp) | Annealing temp (°C) | Final concn (nM) | Target | Reference |
|---|---|---|---|---|---|---|
| Pig-2-Bac | ||||||
| Pig-2-Bac41F | GCATGAATTTAGCTTGCTAAATTTGAT | 116 | 60 | 300 | Pig-specific Bacteroidales | Mieszkin et al. (27) |
| Pig-2-Bac163Rm | ACCTCATACGGTATTAATCCGC | 300 | ||||
| Pig-2Bac113MGB | (VIC)TCCACGGGATAGCC(NFQ-MGB)a | 200 | ||||
| Lactobacilli | ||||||
| LAC1 | AGCAGTAGGGAATCTTCCA | 320 | 60 | 200 | Lactobacilli | Su et al. (37) |
| Lab0677 | CACCGCTACACATGGAG | 200 | ||||
| L. sobrius/amylovorus | ||||||
| OTU171_RDA_F | TTCTGCCTTTTTGGGATCAA | 175 | 60 | 200 | L. sobrius/amylovorus | Konstantinov et al. (21) |
| OTU171_RDA_R | CCTTGTTTATTCAAGTGGGTGA | 200 | ||||
| E. coli | ||||||
| IEC-UP | CAATTTTCGTGTCCCCTTCG | 450 | 58 | 350 | E. coli | Khan et al. (15) |
| IEC-DN | GTTATTGATAGTGTGTCGAAA | 350 |
a
NFQ-MGB, nonfluorescent quencher-minor groove binder.