FIG. 4.

Screening of the transposon library for biofilm-deficient mutants. Strains were incubated statically in LBNS broth for 3 days at 26°C, and the biofilm was quantified by the CV method. Data represent the OD₅₉₅ values of the different strains relative to that of the wt strain, which was set as 100%. Average data of three independent experiments are presented. The biofilm masses (CV values) of all mutants were significantly smaller than that of the wt (P < 0.0001). The error bars represent the standard errors of the means.