TABLE 1.
Primers used in this study
| Primer | Sequencea | Source | nt position |
|---|---|---|---|
| Km-F | 5′-GGAGAAAACTCACCGAGGC-3′ | Km resistance cassette from Tn10 | 647-665b |
| Km-R | 5′- GTCGGGCAATCAGGTGCG-3′ | Km resistance cassette from Tn10 | 1255-1272b |
| Tn-F | 5′-AACGCAGACCGTTCCGTGG-3′ | Mini-Tn10 sequence | 1535-1553b |
| Tn-R | 5′-TCGCTCAGGCGCAATCACG-3′ | Mini-Tn10 sequence | 923-905b |
| A59-F | 5′-TGGTCGTCCGCTGGTTGATGATTA-3′ | E. coli O157:H7, ORF ECs0265 | 351-374c |
| A59-R | 5′-CGGAACATTCATCAGTGTAA-3′ | Mini-Tn10 sequence | 160-141b |
| C24-F | 5′-TGCTGCTTTCATTTGTTCCGG-3′ | E. coli O157:H7, ORF ECs1413 | 344-364c |
| C24-R | 5′-TGGCTCCCTCACTTTCTGGCT-3′ | Tn10 sequence | 332-352b |
| crl-F | 5′-GGGGATATC TGATAATAATAAGGAGTATTGATTATGACGTTACCGAGTGGACA-3′ | E. coli O157:H7, ORF ECs0267 | 1-20d |
| crl-R | 5′-GGGGTCGACTCAGTGATGGTGATGGTGATGCGCCGTTAACTTCACCGGCTCGTCA-3′ | E. coli O157:H7, ORF ECs0267 | 402-377c |
| curli-F | 5′-GGGGATATCTGATAATAATAAGGAGTATTGATTATGCGTGTCAAACATGCAGT-3′ | E. coli O157:H7, ORF ECs1415 | 1-20c |
| curli-R | 5′-GGGGTCGACGGGGCACTCACGCTTTCGCTTAAACAGTAAATGCCGGATTATT-3′ | E. coli O157:H7, intergenic region between ORF ECs1414 and ORF ECs1413 | 1459990-1459928c |
| pBR-F | 5′-AAATCTAACAATGCGCT-3′ | GenBank/EMBL accession no. J01749 | 89-105d |
| pBR-R | 5′-CACCTGTCCTACGAGTTGC-3′ | GenBank/EMBL accession no. J01749 | 751-770d |
a
The EcoRV restriction site is underlined. The SalI restriction site is in bold.
b
Tn10 minitransposon sequence (GenBank accession number AJ601386).
c
E. coli O157:H7 Sakai, Genome Information Research Center, Osaka University, Japan (http://genome.gen-info.osaka-u.ac.jp/bacteria/o157/viewer.html).
d
pBR322 sequence (GenBank accession number J01749).