FIG. 2.

Metabolites in culture fluid of A. nidulans grown under normoxic and hypoxic conditions. A. nidulans WT mycelia (1 g [wet weight]) precultured in MMDN medium were transferred to 500-ml Erlenmeyer flasks containing 100 ml of MMEN medium using ethanol as the sole carbon source and incubated at 30°C under normoxic (open) and hypoxic (closed) conditions. Amino acids in the medium: (A) Ala (circles), Glu (squares), and lactate (triangles); (B) Val (circles), Leu (squares), and Ile (triangles). (C) Intracellular pyruvate cultured under normoxic (open) and hypoxic (closed) conditions. Pyruvate levels in the medium were very low. Data are means of results of three experiments. The standard deviation was <20%.