FIG. 5.

Effect of glucose on branched-chain amino acid production. A. nidulans WT (1 g wet weight) precultured in MMDN medium was transferred to 500-ml Erlenmeyer flasks containing 100 ml of MMEN medium (closed bars) or 100 ml of MMDN medium (open bars) and incubated at 30°C for 12 h under hypoxic conditions. (A) Ammonium, ethanol, and lactate production in culture medium and cell-free lactate dehydrogenase (LDH) activity. *, unable to determine due to vigorous consumption of ethanol added as a carbon source. (B) Branched-chain amino acid production in culture medium. Experiments were repeated three times. Mean values with standard deviations are shown.