TABLE 1.
Intracellular and extracellular metabolites from A. nidulans cultured under normoxic and hypoxic conditionsa
| Aeration | Metabolites (μmol) |
||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Organic acids |
Amino acids |
||||||||||||||
| Lactate | Malonate | Maleate | Succinate | Citrate | Malate | Ala | Glu | Val | Leu | Ile | Thr | Pro | Asp | Phe | |
| Extracellular fraction | |||||||||||||||
| Normoxia | <0.1 | 28 | 10 | 11 | <0.1 | <0.1 | <0.1 | <0.1 | <0.1 | <0.1 | <0.1 | <0.1 | <0.1 | <0.1 | <0.1 |
| Hypoxia | 19 | 7.4 | <0.1 | <0.1 | <0.1 | <0.1 | 61 | 34 | 10 | 18 | 15 | 5.2 | 6.4 | 2.3 | 2.1 |
| Intracellular fraction | |||||||||||||||
| Normoxia | <0.1 | 5.6 | 3.6 | 4.3 | 5.2 | 7.5 | 34 | 40 | 5.3 | 8.1 | 7.9 | 2.6 | 3.6 | 2.6 | 11 |
| Hypoxia | 2.3 | 6.1 | 3.2 | 4.1 | 6.2 | 9.4 | 43 | 47 | 7.0 | 11 | 9.2 | 3.0 | 4.4 | 2.9 | 10 |
a
Mycelia (1 g wet weight) were incubated in 500-ml Erlenmeyer flasks containing 100 ml of MMEN medium using ethanol as the sole carbon source for 12 h at 30°C under normoxic and hypoxic conditions, and intracellular and extracellular metabolites were measured. Pyruvate was undetectable. Data are means of results of three experiments. Standard errors were <20%.