TABLE 2.
Expression of genes involved in branched-chain amino acid and glutamate biosynthesisa
| Predicted gene product | Gene IDb | Relative expression ratio |
||
|---|---|---|---|---|
| Ethanol |
Glucose (12 h) | |||
| 6 h | 12 h | |||
| Acetohydroxy acid synthase small subunit (AHASS) | AN4430.3 | 2.6 | 4.3 | 1.2 |
| Acetohydroxy acid synthase large subunit (AHASL) | AN4956.3 | 1.4 | 2.1 | 1.2 |
| Acetohydroxy acid isomeroreductase (AHAIR) | AN2526.3 | 3.9 | 4.4 | 2.2 |
| Dihydroxy acid dehydratase (DHAD1) | AN6346.3 | 2.4 | 2.2 | 1.5 |
| Dihydroxy acid dehydratase (DHAD2) | AN4058.3 | 1.1 | 1.3 | 1.2 |
| Dihydroxy acid dehydratase (DHAD3) | AN5138.3 | 6.0 | 5.3 | 1.1 |
| Dihydroxy acid dehydratase (DHAD4) | AN7358.3 | 1.4 | 1.6 | 2.0 |
| Transaminase B1 | AN4323.3 | 1.2 | 1.3 | 1.1 |
| Transaminase B2 | AN7878.3 | 2.3 | 5.2 | 1.0 |
| Transaminase B3 | AN5957.3 | 1.9 | 3.1 | 1.2 |
| Transaminase B4 | AN0385.3 | 13.7 | 6.0 | 1.1 |
| Transaminase B5 | AN7876.3 | 2.3 | 4.1 | 2.3 |
| Transaminase B6 | AN8511.3 | 1.1 | 1.1 | 1.0 |
| Glutamate dehydrogenase | AN4376.3 | 1.7 | 6.9 | 2.5 |
| NAD(P) transhydrogenase | AN8130.3 | 3.3 | 3.9 | 3.1 |
a
Transcripts were quantified by real-time PCR using total RNA prepared from A. nidulans cultured at 30°C for 6 and 12 h under normoxic and hypoxic conditions. Ethanol and glucose served as carbon sources. Data are normalized to the β-actin (actA) transcript and are shown as relative expression ratios (hypoxia versus normoxia). Data are means of results of three experiments. Standard deviations were <25%. P < 0.05.
b
Gene IDs are according to the A. nidulans genome database (http://www.broadinstitute.org/annotation/genome/aspergillus_group/MultiHome.html).