TABLE 3.
Enzyme activities in wild type and mutants grown under normoxic and hypoxic conditionsa
Strain | Sp act (nmol min−1 mg−1) |
|||||
---|---|---|---|---|---|---|
AHAS |
Transaminase B |
GDH |
||||
N | H | N | H | N | H | |
WT | 40 ± 8 | 73 ± 10 | 14 ± 3 | 48 ± 6 | 23 ± 4 | 57 ± 12 |
DAHAS-L | <1 | <1 | 11 ± 2 | 38 ± 5 | 20 ± 4 | 48 ± 11 |
DAHAS-S | 30 ± 6 | 39 ± 7 | 15 ± 4 | 52 ± 4 | 26 ± 4 | 64 ± 11 |
DGDH | 43 ± 9 | 73 ± 13 | 17 ± 4 | 56 ± 7 | <0.1 | <0.1 |
Mycelia were incubated in MM medium for 12 h under normoxic (N) and hypoxic (H) conditions. Valine, leucine, and isoleucine (10 mM each) were added to the medium to preculture DAHAS-L. Data are means of results of three experiments ± standard errors.