TABLE 2.

Mutations in the reporter strains

Strain	Sequencea	Mutation	Primers
NZ7100	ATTGATTATATC AAG TGG GAT ATGAAC	Wild typeb
NZ1801	ATTGATTATATC TAG TGG GAT ATGAAC	Substitution stop codon	1L, 1R
NZ1802	ATTGATTATATC AAG TGG TAT ATGAAC	Substitution Asp → Tyr	2L, 2R
NZ1803	ATTGATTATATC AAG GG GAT ATGAAC	1-bp deletion frameshift	3L, 3R
NZ1804	ATTGATTATATC AAG G GAT ATGAAC	2-bp deletion frameshift	4L, 4R
NZ1805	ATTGATTATATC GAAG TGG GAT ATGAAC	1-bp insertion frameshift	5L, 5R
NZ1806	ATTGATTATATC AAG TGG CTG ATATGAAC	2-bp insertion frameshift	6L, 6R
NZ7100	AAGTTACCA GGA GGC TTAGCGGATATTAG	Wild typec
NZ1807	AAGTTACCA GGG GGGG TTAGCGGATATTAG	1-bp insertion frameshift	7L, 7R

^aUnderlining indicates the catalytic site of the melA gene; bold type indicates the mutation site.

^bPositions 1438 to 1464 of the melA gene.

^cPositions 1189 to 1216 of the melA gene.