FIG. 4.

(A and B) T-RFLP profiles generated with the RE HaeIII, from a QRS soil microcosm experiment, showing the similarity of an H₂-treated soil T-RFLP profile (A) to an air-treated soil T-RFLP profile (B). The scale bar at the top indicates the length of the T-RFs in nucleotides (nt), and the markings on the y axes of the profiles indicate relative fluorescence units. The T-RF marked with an asterisk in panel A is 223 nt long. (C) Mean contribution of each T-RF to all of the air-treated QRS soil T-RFLP profiles (n = 8) and all of the H₂-treated QRS soil T-RFLP profiles (n = 9), generated with the RE HaeIII. The mean contributions of each T-RF generated from air-treated samples (white bars) are superimposed on the mean contributions of each T-RF generated from the H₂-treated samples (black bars). The T-RF at 223 nt, which was a significantly greater proportion of the HaeIII T-RFLP profiles generated from the H₂-treated QRS soil (Table 1), is indicated.