TABLE 1.
Mean contribution of T-RFs that constituted a significantly greater proportion of the T-RFLP profiles from the H2-treated microcosms
| RE | Result fora: |
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|---|---|---|---|---|---|---|---|---|---|---|
| GES soil |
QRS soil |
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| H2-specific T-RF length (nt) | % Go (n = 4) | % Ga (n = 10) | % Gh (n = 10) | P value (Ga vs Gh) | H2-specific T-RF length (nt) | % Qo (n = 3) | % Qa (n = 8) | % Qh (n = 9) | P value (Qa vs Qh) | |
| BstUI | 223 | 3.7 | 4.1 | 12.0 | 0.0011 | 220 | 2.5 | 3.5 | 16.7 | 0.0014 |
| HaeIII | 65 | 3.6 | 4.1 | 8.5 | 0.0091 | 223b | 0.6 | 0.3 | 14.7 | 0.0009 |
| HhaI | 358 | 2.6 | 6.6 | 14.6 | 0.0112 | 177 | 0.03 | 0.02 | 9.2 | 0.0002 |
| HinfI | 323 | 17.8 | 16.6 | 26.8 | 0.0169 | 166 | 0.1 | 0.08 | 5.5 | 0.0010 |
| MspI | 140 | 2.1 | 3.0 | 6.0 | 0.0538 | 158 | 2.3 | 2.0 | 10.7 | 0.0044 |
| Sau96I | 64 | 2.1 | 3.0 | 6.1 | 0.0428 | 314 | 0 | 0 | 13.1 | 0.0017 |
The contributions are shown by soil type (where G represents GES soil microcosms and Q represents QRS soil microcosms) and treatment (where o represents original soil, a represents air-treated soil, and h represents H2-treated soil). The results of two-tailed t tests between the T-RFs' contributions to the treated samples from each soil are presented. n indicates the number of T-RFLP profiles analyzed for each soil and treatment for all six REs. In each case, the percentage shown represents the mean contribution of the T-RF to the total area of the T-RFLP profiles.
Comparisons of the HaeIII T-RFLP profiles generated from QRS soil are shown in Fig. 4.