TABLE 2.

Intra-and interassay variability of plasmid standard^a

Copy no. of plasmid standard	BKPyV		JCPyV		Adenovirus
	Mean CT	%CV	Mean CT	%CV	Mean CT	%CV
Intra-assay (n = 10)
5 × 107	15.15	4.43	14.89	1.78	15.21	1.80
5 × 106	18.80	2.04	18.30	1.44	18.49	1.52
5 × 105	22.44	3.52	21.63	1.09	22.01	0.89
5 × 104	24.77	0.95	24.52	1.55	24.51	1.96
5 × 103	28.33	1.48	27.71	0.91	28.14	0.92
5 × 102	32.61	1.83	31.03	1.21	31.56	1.44
5 × 10	35.12	2.00	34.23	1.37	34.64	1.12
5	40.04	13.40	37.97	11.61	40.23	13.00
Interassay (n = 20)b
5 × 107	15.26	4.63	15.03	2.68	15.18	3.22
5 × 106	18.67	2.20	18.22	1.39	18.51	1.36
5 × 105	22.30	3.44	21.68	1.78	22.04	2.68
5 × 104	25.19	3.51	25.03	1.54	25.25	2.04
5 × 103	28.59	1.25	28.22	0.91	28.70	1.55
5 × 102	32.29	1.29	31.56	0.85	32.09	1.12
5 × 10	35.86	2.16	35.09	1.81	35.49	1.57
5	43.23	13.17	42.72	14.58	44.73	13.48

^aC_T, threshold cycle; %CV, percent coefficient of variation.

^bFive replicates, four times.