TABLE 3.
MLST of C. difficile from ELISA-negative stool samples determined both directly using total stool DNA and using isolates cultured from the same stools
| Sample no.a | MLST PCR result (direct) | Direct STd | Culture resultg | Culture STe | PCR ribotype | PaLoc PCR result | lok1/lok3 PCR |
|---|---|---|---|---|---|---|---|
| 22− | + | 6 | + | 6 | 005 | + | − |
| 23− | + | 15 | + | 15 | 010 | − | +f |
| 36− | + | 6 | + | 6 | 005 | + | − |
| 43− | + | 8 | + | 8 | 002 | + | − |
| 69− | + | 2 | + | 2 | 020 | + | − |
| 79− | + | 2 | + | 2 | 020 | + | − |
| 15− | − | + | 69 | 072 | + | − | |
| 55−b | − | + | 1 | 027 | + | − | |
| 85−c | − | + | 1 | 027 | + | − | |
| 99− | − | ++ | 15 | 010 | − | +f | |
| 108− | − | + | 8 | 002 | + | − |
The minus sign indicates an ELISA-negative result.
Patient provided a subsequent sample not included in the study (+7 days) that was ELISA positive and ST-1 by culture (sample not typed by direct MLST).
Patient provided a subsequent sample not included in the study (+39 days) that was ELISA positive and ST-1 by culture (sample not typed by direct MLST).
MLST was performed directly from stool samples.
MLST was performed from cultured isolates.
PCR yielded a769-bp amplicon, confirming the absence of the PaLoc.
Crude estimate of viable C. difficile load. +, <100 colonies; ++, 100 to 1,000 colonies; +++, >1,000 colonies.