TABLE 1.
Comparison of cytospin-enhanced DFA and real-time TaqMan RT-PCR for swine origin influenza virus (S-OIV)
| Swine influenza A virus RT-PCR result | No. of samples with the following cytospin-DFA result for influenza A virus: |
Total no. of samples | ||
|---|---|---|---|---|
| Positive | Negative | Indeterminatea | ||
| Positive | 230 | 33 | 9 | 272 |
| Negative | 2b | 275c | 8 | 285 |
| Total no. of samples | 232 | 310 | 17 | 557 |
a
Atypical staining requiring confirmation by PCR or culture.
b
Two hospitalized children, one 12-year-old child with pneumonia and one 9-month-old child with bronchiolitis, had 1 or 2 influenza virus-positive cells by DFA.
c
SimulFluor cytospin-DFA also detected 17 parainfluenza infections, 2 RSV infections, and 1 adenovirus infection. The sensitivity, specificity, positive predictive value, and negative predictive value for cytospin-DFA were 84.6%, 99.3%, 99.1%, and 87.2%, respectively.