FIG. 2.
Incorporation of C3 on surface-bound activators of the three described complement activation pathways: CP, AP, and LP. pNHS was diluted 1:1 in dilution buffer in the presence of final SPS concentrations from 0 to 1 mg/ml. Afterward, the samples were further diluted 1:5 equivalent to a final serum concentration of 10%, and assays were carried out as described in Materials and Methods. Incorporation of C3 was determined as OD490-650 units. Error bars indicate two times the standard deviation of double determinations.