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. 2010 Jan 20;48(3):928–940. doi: 10.1128/JCM.02045-09

FIG. 3.

FIG. 3.

Results obtained using inactivated influenza viruses in NA inhibition assays. (A) The NA activity increases after 1% Triton X-100 treatment but is subsequently reduced by column-based detergent removal. Lines connect results from experiments with the same virus strain. (B) Effects of 1% Triton X-100 and formalin treatments on the performance of the MUNANA NA inhibition assay with oseltamivir-sensitive and -resistant influenza viruses. Results obtained after column-based detergent removal (Triton + column) are indicated. RFU, relative fluorescence units. (C) IC50 values obtained with the MUNANA assay (for live and Triton X-100-treated virus samples, virus samples treated with Triton X-100 and subjected to detergent removal, and formalin-treated virus samples) and the NA-Star assay (for live and Triton X-100-treated virus samples and virus samples treated with Triton X-100 and subjected to detergent removal) for NA inhibition with oseltamivir-sensitive and -resistant seasonal influenza A(H1N1) viruses. (D) NAI susceptibilities of 12 1% Triton X-100-treated influenza A(H1N1)v virus isolates as measured with the MUNANA and NA-Star NA inhibition assays.