TABLE 3.

Oligonucleotide primers for M-PCR for species identification of coagulase-positive staphylococci and S. schleiferi subsp. schleiferi

Primer	Sequence (5′-3′)	Size of PCR product (bp)	Species
au-F3	TCGCTTGCTATGATTGTGG	359	S. aureus
au-nucR	GCCAATGTTCTACCATAGC
in-F	CATGTCATATTATTGCGAATGA	430	S. intermedius
in-R3	AGGACCATCACCATTGACATATTGAAACC
sch-F	AATGGCTACAATGATAATCACTAA	526	S. schleiferi subsp. coagulans
sch-R	CATATCTGTCTTTCGGCGCG		S. schleiferi subsp. schleiferi
dea-F	TGAAGGCATATTGTAGAACAA	661	S. delphini group A
dea-R	CGRTACTTTTCGTTAGGTCG
hy-F1	CATTATATGATTTGAACGTG	793	S. hyicus
hy-R1	GAATCAATATCGTAAAGTTGC
pse-F2	TRGGCAGTAGGATTCGTTAA	926	S. pseudintermedius
pse-R5	CTTTTGTGCTYCMTTTTGG
deb-F	GGAAGRTTCGTTTTTCCTAGAC	1,135	S. delphini group B
deb-R4	TATGCGATTCAAGAACTGA