Shrinkage and loss of kDNA during RNAi of PRI1. Cells at 0, 3, and 6 days of RNAi were stained with DAPI and observed with a fluorescence microscope. Upper panels, phase contrast; lower panels, DAPI fluorescence. N, nucleus; k, kinetoplast; z, zoid (anucleate cell). Bar, 10 μm.