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. 2010 Jan 11;30(6):1495–1507. doi: 10.1128/MCB.00710-09

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FIG. 5. — Interaction of DUE-B, Cdc45, and TopBP1. (A) Immunoprecipitation (IP) of HeLa cell extracts using normal rabbit serum (NRS, lane 1) or antibody against DUE-B (lane 2), Cdc45 (lane 3), or TopBP1 (lane 4). Proteins indicated to the right of the figure were visualized by Western blotting (WB) with the cognate antibodies. (B) Western blot of lysates (input) of Sf9 cells infected pairwise with baculoviruses expressing DUE-B, TopBP1, and Cdc45 (lanes 1 to 3, unfilled ovals). Lane 4, IP with preimmune IgG; lanes 5 to 10, IP with antibody (filled ovals) against one of each pair of baculovirus-expressed proteins. (C) Western blot of immunoprecipitates of lysates (input) of Sf9 cells infected pairwise with baculoviruses expressing ΔCT DUE-B, TopBP1, and Cdc45 (lanes 1 and 4, unfilled ovals). Lanes 2, 3, 5, and 6, IP with antibody (filled ovals) against one of each pair of baculovirus-expressed proteins.