FIG. 2.

Comparison of association of RECQ1 and RECQ4 helicases with the lamin B2 origin in untreated versus HU-treated cells. T98G cells were treated with 2 mM HU for 24 h. The bar graph shows the quantification of cross-linked lamin B2 origin DNA immunoprecipitated from T98G glioblastoma cells, using antibodies specific to the proteins shown across the bottom. Fold enrichments of origin sequences were determined versus nonorigin control sequences, and the dashed line indicates the threshold enrichment level obtained by using a negative-control antibody (normal rabbit IgG). Results are reported as means ± SEM for at least three independent experiments.