TABLE 3.
P-element insertion mutant lines used and their survival rate after septic infection of E. carotovora subsp. carotovora 15
Strain or mutant no. | Symbol | GenExel no. | Survival rate (%) on day: |
||||
---|---|---|---|---|---|---|---|
0 | 1 | 2 | 3 | 4 | |||
W1118 | Wild type | 100.00 | 98.47 | 96.71 | 94.86 | 93.99 | |
Imd1 | 100.00 | 43.06 | 28.89 | 22.59 | 16.94 | ||
RelE20 | 100.00 | 91.17 | 50.04 | 17.14 | 13.90 | ||
1 | Perioda | G10561 | 100.00 | 95.99 | 95.99 | 77.66 | 68.65 |
2 | Fimbrin (CG8649) | G10929 | 100.00 | 98.33 | 96.61 | 90.50 | 88.83 |
3 | CG3881 | G11876 | 100.00 | 97.33 | 96.67 | 94.67 | 94.00 |
4 | CycE | G12107 | 100.00 | 98.72 | 98.72 | 98.72 | 98.72 |
5 | Cdk4 | G12455 | 100.00 | 95.71 | 94.87 | 92.24 | 90.45 |
6 | CG6860 | G12512 | 100.00 | 95.83 | 90.83 | 88.33 | 87.50 |
7 | l(3)05822 | G13184 | 100.00 | 96.67 | 95.00 | 92.20 | 90.53 |
8 | G-ialpha65A | G13928 | 100.00 | 97.37 | 96.87 | 95.05 | 92.89 |
9 | AnnIX | G13932 | 100.00 | 97.70 | 97.70 | 97.70 | 97.70 |
10 | Puromycin-sensitive aminopeptidase | G13991 | 100.00 | 94.00 | 91.56 | 91.56 | 89.33 |
11 | Pak3 | G14159 | 100.00 | 96.11 | 92.70 | 90.95 | 87.58 |
12 | Non-claret disjunctional | G14231 | 100.00 | 98.64 | 94.62 | 93.29 | 91.26 |
13 | Mo25 | G14716 | 100.00 | 96.67 | 95.56 | 93.33 | 92.22 |
14 | RhoLa | G15295 | 100.00 | 93.83 | 91.84 | 81.20 | 69.75 |
15 | Ack | G16320 | 100.00 | 95.00 | 92.50 | 90.83 | 90.00 |
16 | EndoA | G16603 | 100.00 | 94.44 | 93.33 | 91.11 | 90.00 |
17 | NetB | G1622 | 100.00 | 99.00 | 97.33 | 97.33 | 96.50 |
18 | String | G16904 | 100.00 | 92.18 | 92.18 | 87.70 | 83.26 |
19 | CG12065 | G17117 | 100.00 | 98.33 | 95.34 | 91.12 | 84.98 |
20 | Inx2 | G17438 | 100.00 | 97.78 | 97.78 | 96.67 | 96.67 |
21 | CG9086 | G17767 | 100.00 | 97.78 | 95.55 | 93.33 | 93.33 |
22 | CG2803 | G18036 | 100.00 | 100.00 | 96.67 | 95.00 | 95.00 |
23 | CG10916 | G18115 | 100.00 | 99.17 | 96.67 | 95.83 | 94.17 |
24 | CG30015 | G18234 | 100.00 | 98.28 | 96.61 | 88.99 | 86.41 |
25 | CG10973 | G18338 | 100.00 | 98.00 | 97.31 | 96.62 | 96.62 |
26 | CG4080 | G18431 | 100.00 | 96.67 | 94.37 | 94.37 | 93.22 |
27 | Trxr-1 | G1853 | 100.00 | 95.56 | 92.96 | 89.26 | 89.26 |
28 | CG31216a | G18713 | 100.00 | 87.04 | 85.50 | 80.70 | 57.93 |
29 | CG8588 | G20048 | 100.00 | 97.78 | 96.67 | 94.44 | 93.33 |
30 | Dap160 | G2320 | 100.00 | 97.19 | 95.11 | 91.63 | 90.30 |
31 | Betaggt-II | G2472 | 100.00 | 94.45 | 90.00 | 85.56 | 81.11 |
32 | Wun | G2520 | 100.00 | 100.00 | 100.00 | 98.89 | 94.45 |
33 | CG11242 | G2655 | 100.00 | 99.33 | 96.55 | 96.55 | 93.14 |
34 | Dcp-1 (CG5370) | G2729 | 100.00 | 96.00 | 93.73 | 92.40 | 90.40 |
35 | CG10376 | G2887 | 100.00 | 98.81 | 96.35 | 95.12 | 91.32 |
36 | CG8445 | G3196 | 100.00 | 98.00 | 95.31 | 91.20 | 87.86 |
37 | Cysteine proteinase 1 | G3277 | 100.00 | 97.74 | 95.56 | 83.33 | 83.33 |
38 | CG17019 | G3302 | 100.00 | 94.14 | 90.80 | 86.58 | 84.05 |
39 | CG7816 | G3896 | 100.00 | 97.78 | 94.48 | 92.71 | 87.86 |
40 | Syx1A | G4126 | 100.00 | 98.89 | 98.89 | 97.78 | 94.44 |
41 | CG31547 | G4171 | 100.00 | 91.38 | 87.16 | 82.10 | 77.82 |
42 | CG17723 | G4288 | 100.00 | 97.78 | 95.38 | 95.38 | 94.10 |
43 | Myo61Fa | G4411 | 100.00 | 75.15 | 67.24 | 57.85 | 53.59 |
44 | CG9796 | G4657 | 100.00 | 92.84 | 82.73 | 74.66 | 74.66 |
45 | Best1 (CG6264) | G4725 | 100.00 | 97.79 | 97.79 | 95.39 | 95.39 |
46 | Darkener of apricot | G4792 | 100.00 | 92.59 | 90.00 | 88.59 | 88.59 |
47 | PGRP-LF | G4841 | 100.00 | 93.74 | 91.64 | 90.34 | 85.32 |
48 | CG9139 | G5077 | 100.00 | 97.34 | 97.34 | 93.60 | 93.60 |
49 | Syx13 | G5399 | 100.00 | 98.33 | 94.17 | 93.33 | 91.64 |
50 | Calpain-B | G5405 | 100.00 | 95.01 | 91.13 | 89.45 | 85.45 |
51 | CG16718 | G5419 | 100.00 | 90.83 | 90.83 | 87.50 | 86.67 |
52 | CG15234 | G5655 | 100.00 | 97.82 | 96.90 | 90.62 | 87.16 |
53 | a | G7875 | 100.00 | 97.61 | 94.27 | 90.60 | 89.49 |
54 | Ptp4E | G773 | 100.00 | 100.00 | 97.50 | 96.67 | 95.00 |
55 | Sktl | G8067 | 100.00 | 92.11 | 89.85 | 85.33 | 80.88 |
56 | Cher | G9093 | 100.00 | 98.48 | 98.15 | 97.78 | 97.78 |
57 | CG6454 | G9545 | 100.00 | 100.00 | 97.37 | 91.72 | 91.72 |
58 | Rab35 | G11110 | 100.00 | 80.00 | 73.33 | 63.33 | 46.67 |
59 | CG12004 | G4815 | 100.00 | 96.90 | 90.06 | 88.15 | 85.54 |
Mutants exhibited significantly higher lethality after infection compared to wild type flies. (P < 0.02).