FIG. 8.

SHP regulates mitochondrial respiration and coupling in hepatocytes. (A to D) Mitochondrial respiratory rates of isolated hepatic mitochondria from 10-week-old WT, SHP KO (SKO), NC, SHP transgenic (STG) livers using succinate (5 mM)-rotenone (10 μM) or palmitoyl-carnitine (20 μM)-malate (5 mM) as substrates (n = 5 mice, triplicate assays of liver mitochondria isolated from each mouse liver). The mice were treated with vehicle (A and B) or APHN (C and D) (10 mg/kg of body weight) for 1 day by oral feeding. Data are represented as mean ± SEM. (E) ATP/O (ATP/state3) ratios with succinate-rotenone and palmitoylcarnitine-malate as substrates in isolated liver mitochondria from WT, SKO, NC, and STG mice treated with vehicle or AHPN. Data are represented as mean ± SEM. (F) Detection of reactive oxygen species (ROS) in Huh7 cells overexpressing an SHP expression plasmid in the absence (DMSO) or presence of AHPN (0.1 μM or 1 μM) or 3-Cl-AHPC (1 μM or 10 μM) for 24 h. The experiments were repeated twice (triplicate plates/time) with similar results. One representative result is shown.