Skip to main content
. 2010 Jan 11;30(6):1467–1477. doi: 10.1128/MCB.01151-09

FIG. 4.

FIG. 4.

Progression of RNAPII through the locus is required for SIR complex displacement from GAL-VPS13-HMR-E. (A) Schematic representation of the GAL-VPS13 locus with FBA1 terminator and HMR-E silencer sequences inserted at 3 kb and 6 kb, respectively. Horizontal lines beneath the gene region indicate the positions of coding region (0.1-, 3.5-, 5.5-, 6.4-, and 8.5-kb) PCR probes used. (B and C) ChIP assay was carried out to determine the density of RNAPII (B) and Sir3 (C) on GAL-VPS13-HMR-E (AKY354) and GAL-VPS13-term3k-HMR-E (AKY612) after transcription induction. The bars indicate the levels of RNAPII in galactose medium relative to the level in cells grown in glucose-containing medium (set as 1) and the level of Sir3 in galactose-containing medium relative to the signal from the coding region of the constitutively expressed FBA1 gene.