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. 2010 Jan 11;30(6):1467–1477. doi: 10.1128/MCB.01151-09

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FIG. 7. — Induction of the GAL-VPS13-HMR-E locus in G₁-arrested cells. (A) GAL-VPS13 (AKY362), (B) GAL-VPS13-HMR-E (AKY354), and (C) GAL-VPS13-HMR-E H3 K56R (AKY399) strains were grown overnight in raffinose, arrested in G₁ by α-factor, and kept arrested during galactose induction. RNA samples for RT-PCR were extracted from cells grown in raffinose (0) and 30, 60, and 180 min after galactose induction and analyzed as described for Fig. 5. Equal loadings were confirmed by the FBA1 mRNA amount from each strain.