FIG. 3.

Effect of JNK deficiency on proliferation. (A) Day 7 EBs were pulse-labeled with BrdU and fixed. Frozen sections were stained with DAPI (4′,6′-diamidino-2-phenylindole) and an antibody to BrdU and then examined by immunofluorescence analysis. Scale bar, 150 μm. (B) The mean fluorescence intensity of the BrdU immunofluorescence was measured by using ImageJ software and is presented as the means ± the SD (n = 100 EBs). Statistically significant differences are indicated (*, P < 0.05; **, P < 0.01).