TABLE 1.
Amplimers used in this study
| Gene | Amplimer pair sequences (5′-3′) |
|
|---|---|---|
| Amplimer 1 | Amplimer 2 | |
| Anf | GGGGGTAGGATTGACAGGAT | CAGAGTGGGAGAGGCAAGAC |
| Apoa2 | CACTGCTGGTAACCATCTG | TCGAGGTTCATTAAACTGCT |
| BMHC | CTACAGGCCTGGGCTTACCT | TCTCCTTCTCAGACTTCCGC |
| Bmp4 | GTTTCCTCTTCAACCTCAGC | TATACGGTGGAAGCCCTGTT |
| Brachyury | CTCACCAACAAGCTCAATGG | ACAGCTATGAACTGGGTCTC |
| Emx2 | AGCTTTTAAGGCTAGAGCAC | CCTGAGTTTCCGTAAGACTG |
| Flk1 | GGCGGTGGTGACAGTATCTT | CTCGGTGATGTACACGATGC |
| Gata4 | TGTCAATTGTGGGGCCATGTC | GTGCCCCAGCCTTTTACTTTG |
| Hnf1 | GAAAGCAACGGGAGATCCTCCGAC | TAGGCATCCATGGCCAGCTTCTGC |
| Hnf3 | TGGTCACTGGGGACAAGGGAA | GCAACAACAGCAATAGAGAAC |
| Keratin17 | GTAGTGCTCTTGGGAGCAACAG | AGAACCAGTCTTCGGCATCCTT |
| MEF2c | ACTGGGAAACCCCAATCTTC | ATCAGACCGCCTGTGTTACC |
| Mlv2v | AAAGAGGCTCCAGGTCCAAT | CCTCTCTGCTTGTGTGGTCA |
| Nkx2.5 | TGCAGAAGGCAGTGGAGCTGGACAAGCC | TTGCACTTGTAGCGACGGTTCTGGAACCAG |
| Nodal | TGGAGCGCATTTGGATGGAGAC | TACTGCTTGGGGTAGATGATCC |
| Otx1 | TTCACGCGCTCACAGCTGGA | ACCAAACCTGGACTCTGGAC |
| Pax2 | TGCCGAATACAAGCGACAGAAC | TGGAAGACATCGGGATAGGAAG |
| Sox17 | GCCAAAGACGAACGCAAGCGG | TCATGCGCTTCACCTGCTTG |
| Transferrin | ATTCTCAAAGTGGCACAGGAACAC | GGGTTCTTTCCTTCGGTGTTATCC |
| Wnt1 | TCTTCGGCAAGATCGTCAACCG | AAGGTTCATGAGGAAGCGTAGG |