FIG. 4.

Complementation of mutants with mutations in genes encoding proteins in the X. campestris pv. campestris N-acetylglucosamine (GlcNAc) utilization pathway. After overnight growth in complete medium, cells were harvested, washed, and resuspended in minimal medium containing 200 μM isopropyl-β-thiogalactoside (IPTG), 5 μg/ml of tetracycline, and 10 mM (A, B, C, and E) or 100 μM (D) GlcNAc. The error bars indicate the standard deviations obtained from 4 independent experiments. WT, wild-type strain.