TABLE 1.
Plasmids and X. campestris pv. campestris strains used or generated in this study
| Strain or plasmid | Characteristicsa | Locationb | Designation | Reference |
|---|---|---|---|---|
| Xanthomonas strains | ||||
| Wild type | Wild-type strain; rifampin-resistant derivative of X. campestris pv. campestris LMG568 (= ATCC 33913) | 31 | ||
| nixB::pVO | XCC0531::pVO155; Rifr Kmr | 736 | XP010 | 7 |
| nixC::pVO | XCC2944::pVO155; Rifr Kmr | 334 | XP041 | 7 |
| nixD::pVO | XCC2887::pVO155; Rifr Kmr | 1558 | XP040 | 7 |
| naxA::pVO | XCC3045::pVO155; Rifr Kmr | 1572 | XP044 | 7 |
| naxB::pVO | XCC3046::pVO155; Rifr Kmr | 702 | XP045 | 7 |
| nixA::pVO | XCC3408::pVO155; Rifr Kmr | 1897 | XP059 | 7 |
| nagQ::pVO | XCC3414::pVO155; Rifr Kmr | 659 | XP108 | This study |
| nagR::pVO | XCC3412::pVO155; Rifr Kmr | 519 | XP109 | This study |
| nagA::pVO | XCC3410::pVO155; Rifr Kmr | 236 | XP110 | This study |
| nagK-IIA::pVO | XCC2886::pVO155; Rifr Kmr | 303 | XP111 | This study |
| ΔnagQ | ΔXCC3414; Rifr | From 203 to stop | XP112 | This study |
| ΔnagR | ΔXCC3412; Rifr | From start to stop | XP113 | This study |
| ΔnagP | ΔXCC3413; Rifr | From start to stop | XP114 | This study |
| ΔnagA | ΔXCC3410; Rifr | From start to stop | XP115 | This study |
| ΔnagB-II | ΔXCC3411; Rifr | From start to stop | XP116 | This study |
| ΔnagK-IIA | ΔXCC2886; Rifr | From start to stop | XP117 | This study |
| ΔnagK-IIB | ΔXCC2943; Rifr | From start to stop | XP118 | This study |
| ΔnagK-IIAB | ΔXCC2886 ΔXCC2943; Rifr | From start to stop | XP119 | This study |
| ΔnagA pC-nagA | ΔXCC3410 pC-XCC3410; Rifr Tetr | XP120 | This study | |
| ΔnagB-II pC-nagB-II | ΔXCC3411 pC-XCC3411; Rifr Tetr | XP121 | This study | |
| ΔnagB-II pC-nagB-II-nagA | ΔXCC3411 pC-XCC3411-XCC3410; Rifr Tetr | XP122 | This study | |
| ΔnagK-IIA pC-nagK-IIA | ΔXCC2886 pC-XCC2886; Rifr Tetr | XP123 | This study | |
| ΔnagK-IIB pC-nagK-IIB | ΔXCC2943 pC-XCC2943; Rifr Tetr | XP124 | This study | |
| ΔnagK-IIAB pC-nagK-IIA | ΔXCC2886 ΔXCC2943 pC-XCC2886; Rifr Tetr | XP125 | This study | |
| ΔnagK-IIAB pC-nagK-IIB | ΔXCC2886 ΔXCC2943 pC-XCC2943; Rifr Tetr | XP126 | This study | |
| ΔnagR nagK-IIA::pVO | ΔXCC3412 XCC2886::pVO155; Rifr Kmr | XP127 | This study | |
| ΔnagQ nagK-IIA::pVO | ΔXCC3414 XCC2886::pVO155; Rifr Kmr | XP128 | This study | |
| ΔnagP nagA::pVO | ΔXCC3413 XCC3410::pVO155; Rifr Kmr | XP129 | This study | |
| ΔnagK-IIAB nagA::pVO | ΔXCC2886 ΔXCC2943 XCC3410::pVO155; Rifr Kmr | XP130 | This study | |
| ΔnagP ΔnagA | ΔXCC3413 ΔXCC3410; Rifr | XP131 | This study | |
| ΔnagK-IIAB ΔnagA | ΔXCC2886 ΔXCC2943 ΔXCC3410; Rifr | XP132 | This study | |
| Plasmids | ||||
| pVO155 | pUC119 derivative containing the promoterless gus (uidA) reporter gene encoding β-glucuronidase, used for insertion mutagenesis; Kmr Ampr | 34 | ||
| pFAJ1700 | pTR102-derived expression vector containing a multiple-cloning site and transcriptional terminators in both orientations; Tetr Ampr | 15 | ||
| pSC150 | pET-26b(+) derivative vector with a Ptac promoter sequence; Kmr | 13 | ||
| pCZ917 | pFAJ1700 derivative containing 2,094 bp of pSC150 with lacI, Ptac promoter, and T7 terminator; Tetr Ampr | This study | ||
| pC-nagA | pCZ917-XCC3410; Tetr Kmr | From −20 to stop | This study | |
| pC-nagB-II | pCZ917-XCC3411; Tetr Kmr | From −19 to stop | This study | |
| pC-nagB-II-nagA | pCZ917-XCC3411-XCC3410; Tetr Kmr | From −19 of XCC3411 to stop of XCC3410 | This study | |
| pC-nagK-IIA | pCZ917-XCC2886; Tetr Kmr | From −21 to stop | This study | |
| pC-nagK-IIB | pCZ917-XCC2943; Tetr Kmr | From −19 to stop | This study |
a
Rif: rifampin; Km: kanamycin; Tet: tetracycline.
b
Position of insertion, deletion, or X. campestris pv. campestris sequence cloned relative to the putative start codon.