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Gel filtration chromatogram of IscA (monomer, 13.7 kDa including His tag; dashed line) and DsrR (monomer, 13.6 kDa including His tag; solid line). The proteins were loaded onto a Superdex-75 column (GE Healthcare) preequilibrated with 50 mM Tris-HCl (pH 8.0), 500 mM NaCl. The flow rate was 0.5 ml min−1. The elution was monitored by absorption at 280 nm. The column had been calibrated with several proteins of known molecular masses.
