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RT-PCR for six SPI genes for ramA::aph (A), pTRChisA-ramA (B), and ramR::aph (C) (asterisks show significant [P < 0.05] differences) and RT-PCR for acrB, tolC, and ramA (D) (all RT-PCR data shown are significant; P < 0.05). Black bars, microarray data; gray bars, RT-PCR data; dashed line, fold change of 1 (i.e., no change); for all array changes, a B value of >0 is considered significant.
