FIG. 2.

Comparison of the mml gene cluster of P. reinekei MT1 with those of C. necator JMP134 and C. necator H16. Genes encoding catabolic enzymes, transcriptional regulators, and putative transporters are indicated in light gray, gray, and dark gray, respectively. Genes framing the mml gene clusters are indicated in black. Genes essential for growth on 4-methylsalicylate are indicated with black triangles, whereas those that are dispensable are indicated with unfilled triangles. Numbers below the arrows indicate the primer pairs utilized to assess transcription of intergenic regions. Double-headed arrows represent intergenic regions transcribed during growth on 4-methylsalicylate (MT1) or 4-methylbenzoate (JMP134::X), and lines indicate those regions not transcribed. Experimentally determined promoter regions are indicated by curved arrows.