TABLE 3.

¹H NMR data of metabolites formed by P .reinekei MT1 deletion mutants^a

^aChemical shift and coupling constants were calculated from representative spectra obtained from supernatants of mutant MT1ΔmmlC (4-methyl-3-oxoadipate) or MT1ΔmmlK (methylsuccinate) after incubation with 4-methylsalicylate or extract of supernatant of mutant MT1ΔmmlL (3-ML) dissolved in acetone-d₆. ¹H NMR data were recorded at 300 MHz. ¹H NMR data previously described for 3-methylmuconolactone recorded at 80 MHz in CDCl₃ (47) (top section) and those of 4-methyl-3-oxoadipate dimethylester in CDCl₃ recorded at 200 MHz (47) (middle section) or of authentic methylsuccinate dissolved in the same medium and recorded at 600 MHz (bottom section) are given in parentheses. Doublets, quartets, and multiplets are abbreviated as d, q and m, respectively.