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. 2010 Jan 14;192(6):1573–1585. doi: 10.1128/JB.01394-09

FIG. 3.

FIG. 3.

lacZ expression under control of the promoters of the stringent operons. The core ptsGHI, pycA, and alsSD promoters (nucleotides −55 to 26), as well as the pdhABCD promoter (nucleotides −47 to 164), were placed upstream of lacZ, and each of the resultant lacZ fusions was integrated into the amyE locus of the wild-type, relA1, and ΔcodY strains. lacZ expression under control of the ptsGHI (A), pdhABCD (B), pycA (C), and alsSD (D) promoters (PptsGHI, PpdhABCD, PpycA, and PalsSD) was monitored using strains FU906, FU1025, and FU1029, strains FU1019, FU1021, and FU1031, strains FU977, and FU1023, and FU1027, and strains FU937, FU1026, and FU1041, respectively, upon lysine starvation and decoyinine treatment, as described in Materials and Methods. OD600 values and LacZ activities are indicated by squares and circles; filled and open symbols indicate treatments with and without lysine starvation and decoyinine, respectively. Arrows indicate the onset of these stresses. In the upper part of panel D, alsSD transcription, whose activation is mediated by AlsR, is shown.