TABLE 2.
DNA microarry analysis of CodY-dependent and -independent stringent control induced by decoyinine addition
| Genea | Ratio of expression with decoyinine to expression without decoyinine in the CodY+ backgroundb | Ratio of expression with decoyinine to expression without decoyinine in the CodY− backgroundb | CodY−/CodY+ expression ratiob,c |
|---|---|---|---|
| alsS | 21 | 7.5 | 1.0 |
| alsD | 13 | 7.8 | 0.54 |
| pdhA | 0.13 | 0.09 | 0.70 |
| pdhB | 0.10 | 0.06 | 0.54 |
| pdhC | 0.13 | 0.16 | 0.73 |
| pdhD | 0.16 | 0.31 | 0.75 |
| ilvB | 4.8 | 1.7 | 38 |
| ilvN | 5.9 | 1.9 | 44 |
| ilvC | 8.0 | 1.9 | 49 |
| leuA | 8.7 | 1.8 | 47 |
| leuB | 15 | 1.6 | 46 |
| leuC | 24 | 1.50 | 46 |
| leuD | NDd | ND | ND |
| ptsG | 0.01 | 0.03 | 0.38 |
| ptsH | 0.60 | 0.24 | 0.81 |
| ptsI | 0.45 | 0.33 | 0.70 |
| pycA | 3.1 | 2.2 | 0.96 |
a
Of the genes that are involved in glucose and pyruvate metabolism (Fig. 1), those whose expression was altered more than 3-fold upon addition of decoyinine in the wild-type background are included. The ptsHI genes are also included.
b
The standard deviations of the expression ratios were less than 15%.
c
The expression ratios were obtained by a DNA microarray analysis performed previously (28).
d
ND, the expression signal of leuD was not detectable due to unknown technical problems with our DNA microarray analysis.