FIG. 6.

Impact of pepD deletion on M. tuberculosis gene expression in the absence or presence of SDS stress. (A and B) Wild-type M. tuberculosis, the ΔpepD mutant, the ΔpepD complemented strain, and the ΔpepD S317A complemented strain were grown in the absence of stress (A) or were exposed to 0.05% SDS for 90 min (B). The relative amounts of sigE, mprA, degP, pepA, rseA, or sigH expression in the strains were then compared. The expression level for each gene under each condition was normalized to that of the 16S ribosomal gene, rrs. The results represent the means ± SEM from three independent experiments performed in triplicate. *, P < 0.05.