FIG. 1.

Growth and survival during anaerobic dormancy determined by recovery on solid and liquid media. Wild-type, DorKO mutant, and complemented bacteria were grown in glass tubes with stir bars and a culture-to-headspace ratio of 0.65. Cultures were stirred with magnetic stirrers. (A) CFU were counted at various times by plating samples from tubes harvested sacrificially. Squares, H37Rv; triangles, DorKO; circles, DorCO. (B) Viability assessed by serial dilution of dormant bacteria in DTA broth using the MPN technique and comparison to CFU values (39). Open bars, H37Rv on plates; bars with horizontal stripes, H37Rv in liquid medium; solid bars, DorKO on plates; checkered bars, DorKO in liquid medium; gray bars, DorCO on plates; stippled bars, DorCO in liquid medium. The values are the averages of three experiments.