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Cytotoxic activity of the gp100-reactive DN (T4H2) and CD8+ (3.9) T cell clone was examined at an effector-to-target (E/T) ratio of 5:1 using indicated concentrations of gp100 peptide loaded onto T2 cells. The cytotoxic capacity of the T cell clones was assessed by a standard 4 h 51Cr-release assay. Shown are mean values of triplicates ±SEM
