Figure 4.

Summary of heteronuclear [¹H]-¹⁵N NOE data used to assess the backbone flexibility of the PrP(29–231) and PrP(90–231). (A) The mean values of heteronuclear NOE from two sets of data are plotted against residue number in the full-length SHa PrP(29–231). The error bars represent the difference between the two data sets. The NOEs in four of the octarepeats (residues 59–91) are approximate values estimated by dividing the degenerate peak volumes by four. (B) Heteronuclear NOE values for PrP(90–231). All spectroscopic parameters and processing procedures are the same as those of PrP(29–231).