A. APC inhibits DNA replication in vitro in a dose-dependent manner. Representative results from an in vitro DNA replication assay. Replicated, labeled DNA is separated by electrophoresis and visualized by autoradiography. The final concentration of protein in each assay is indicated above the autoradiogram.
B. Schematic diagram of four nonoverlapping APC segments containing unique amino acids and functional domains.
C. The carboxyl-terminus of APC inhibits DNA replication in vitro. Representative results from an in vitro DNA replication assay. Replicated, labeled DNA is separated by electrophoresis and visualized by autoradiography. The final concentration of proteins in each assay was 200 nM.