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Purification of UAF. (A) Scheme for purification of UAF. (B) A silver-stained gel of a peak fraction from the final column. The polypeptide components of UAF previously identified (4) are indicated. Aprotinin added as carrier for TCA precipitation appears as a heavy band at the bottom of the gel. A band just above this heavy band may represent histone H4 (see Discussion). Separation was in a 10–15% SDS/pHast gel.
