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. 2010 Mar 5;6(3):e1000799. doi: 10.1371/journal.ppat.1000799

Figure 7. Tethering of ORF57 to CMV-Δ1 restores ORF57-responsiveness.

Figure 7

Top, northern blots showing levels of the PAN RNA and a control signal from a representative experiment. Fl-ORF57, MS2-NLS-Fl-ORF57, and MS2-NLS-Fl were co-transfected as indicated. The NLS was included to ensure that the MS2 control was appropriately localized to the nucleus. Because the MS2-NLS-Fl-ORF57 expresses at lower levels than Fl-ORF57 (data not shown), the transfection conditions were altered from previous experiments (see Materials and Methods). Average values from three experiments with standard deviations are shown below. The black bars (lanes 1–8), light gray bars (lanes 9–16), and the dark gray bars (lanes 17–24) show relative RNA levels expressed from the CMV-WT, the CMV-Δ1-XMS2, and the CMV-Δ1 plasmid, respectively. These constructs are drawn below (not to scale). In each experiment, the results were normalized to the average value of the “no ORF57” control, which was performed in duplicate (lanes 1,8; 9,16; and 17, 24).