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. Author manuscript; available in PMC: 2011 Mar 30.
Published in final edited form as: J Neurosci Methods. 2010 Jan 11;187(2):156–166. doi: 10.1016/j.jneumeth.2010.01.006

Figure 5.

Figure 5

Repeated measurements before and after fixation. Eyes were weighed and axis lengths measured over time before and after fixation with 10% buffered formalin. C57BL/6J mice were sacrificed at P60 (N=10). The left eye (OS) was enucleated first; fat was trimmed from whole eye globe; eye weight was taken; and then axis lengths were measured. After 3 measurements, the eye was placed in 10% buffered formalin for 1 h. The eye was then re-weighed 3 more times. Average time to reach measurement 3 was 4.73 min ± 0.467 min. Average time from measurement 4 to 6 was 4.60 min ± 0.699 min. Error bars represent standard deviations. Panel A: Eye weights over time. Panel B: Superior-inferior over time. Regardless of axes or weight, the same initial pattern of evaporation as before emerged. However, eyes could not be rehydrated after fixation with formalin.