Figure 6.

Pharmacological inhibition of Gsk3 decreases the level of APC at microtubule plus-ends of DRG neurons from wild-type or Dvl1 mutant mice. A, In DRG neurons from wild-type mice, the Gsk3 inhibitor Bio induces the loss of APC from MT tips at the periphery of the growth cone (arrowheads). Similarly, DRG neurons from Dvl1 mutant mice show a decrease in the level of APC at the tips of MTs compared with control neurons (arrowheads). Scale bar, 15 μm. B, DRG neurons from Dvl1 mutant mice do not show a decrease in the number of MT plus-ends containing APC after Wnt3a treatment compared with untreated neurons. C, Quantification shows that Gsk3 inhibition with Bio increases growth cone (GC) size to the same extent on DRG neurons from wild-type or Dvl1 mutant mice. D, Quantification shows that the percentage of MT tips with APC per growth cone decreases similarly in wild-type or Dvl1 mutant DRG neurons when exposed to Bio. Values are mean ± SEM of at least three independent experiments. ***p < 0.001. n = 100.